Suppression of Methionine-Induced Hyperhomocysteinemia by Dietary Eritadenine in Rats
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The effect of dietary eritadenine on the plasma homocysteine concentration was investigated in methionine-induced hyperhomocysteinemic rats. The rats were fed on the control or eritadenine-supplemented (50 mg/kg) diet for 10 d. The animals were then injected with saline or methionine at a level of 100 or 300 mg/kg of body weight, and sacrificed 2 h or a more appropriate time after injection. The methionine injection increased the post-2 h concentration of plasma homocysteine in a dose-dependent manner in the control rats, this increase being significantly suppressed in the eritadenine-fed rats. This effect persisted up to 8 h after the methionine injection. The hepatic concentrations of <I>S</I>-adenosylmethionine and <I>S</I>-adenosylhomocysteine were increased by eritadenine, whereas the hepatic homocysteine concentration was inversely decreased. The cystathionine β-synthase activity in the liver was increased by eritadenine. It is suggested from these results that eritadenine might suppress the methionine-induced increase in plasma homocysteine concentration by dual mechanisms: slowing the homocysteine production from <I>S</I>-adenosylhomocysteine and increasing the removal of homocysteine due to the enhanced activity of cystathionine β-synthase.
- Agricultural and Biological Chemistry
Agricultural and Biological Chemistry 70(8), 1987-1991, 2006-08-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry