Reverse Transcriptase-Like Sequences Related to Retrotransposon in a Red Alga, Porphyra yezoensis
Four DNA fragments encoding a reverse transcriptase (RT)-like gene related to that of long terminal repeat (LTR) retrotransposons were isolated from the red alga <I>Porphyra yezoensis</I> by genomic PCR. Southern blot analysis suggested that one clone exists as a single copy per genome. Its full-length cDNA (<I>PyRE2A</I>) contained RT/RNase H-like sequences, which are most closely related to those of the <I>Volvox</I> LTR retrotransposon, although two stop codons were present within the RT region. We did not find any sequence related to LTR retrotransposons other than RT/RNase H in <I>RyRE2A</I>. These results indicate that <I>PyRE2A</I> is a single RT/RNase H-like gene and a defective progenitor of LTR retrotransposons.
- Bioscience, biotechnology, and biochemistry
Bioscience, biotechnology, and biochemistry 70(8), 1999-2003, 2006-08-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry