Induction of Resistance against Rice Blast Fungus in Rice Plants Treated with a Potent Elicitor, N-Acetylchitooligosaccharide

  • TANABE Shigeru
    Department of Biochemistry, National Institute of Agrobiological Sciences
  • OKADA Mitsuo
    Department of Biochemistry, National Institute of Agrobiological Sciences
  • JIKUMARU Yusuke
    Biotechnology Research Center, The University of Tokyo
  • YAMANE Hisakazu
    Biotechnology Research Center, The University of Tokyo
  • KAKU Hanae
    Department of Life Sciences, Faculty of Agriculture, Meiji University
  • SHIBUYA Naoto
    Department of Life Sciences, Faculty of Agriculture, Meiji University
  • MINAMI Eiichi
    Department of Biochemistry, National Institute of Agrobiological Sciences

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  • Induction of Resistance against Rice Blast Fungus in Rice Plants Treated with a Potent Elicitor,<i>N</i>-Acetylchitooligosaccharide

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Abstract

The mode of action of a potent elicitor, N-acetylchitooligosaccharide, in rice plants was examined. In intact seedlings, no significant uptake of the elicitor via the roots was observed within 3 h, whereas rapid uptake was observed in excised leaves. Rapid and transient expression of an elicitor-responsive gene, EL2, was induced in the leaves of intact seedlings sprayed with the elicitor or in the roots and leaves of intact seedlings by immersing roots in the elicitor solution. Histochemical analysis indicated that EL2 was expressed in cells exposed to the elicitor of root and leaves. In seedlings treated with the elicitor for 1 d or longer, hyphal growth of rice blast fungus was significantly delayed, and an accumulation of auto-fluorescence around the infection site was observed. Two defense-related genes, PR-1 and PR-10 (PBZ1), were induced in a systemic and local manner by elicitor treatment, in correlation with the induction of resistance against rice blast fungus. N-Acetylchitoheptaose did not inhibit the hyphal growth of the fungi. These results indicate the occurrence of systemic signal transmission from N-acetylchitooligosaccharide in rice plants.

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