Bacillus subtilis DEAD Protein YdbR Possesses ATPase, RNA Binding, and RNA Unwinding Activities

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The product of an open reading frame (ORF) (called YdbR) identified while analyzing the <I>Bacillus subtilis</I> genome has been classified as an Asp-Glu-Ala-Asp (DEAD) protein, but the biological function and enzymology of YdbR have not been characterized in detail. Here we show that recombinant YdbR-His<SUB>6</SUB> purified from <I>Escherichia coli</I> is an ATP-independent RNA binding protein. It also possesses RNA-dependent ATPase activity stimulated not only by total RNA from <I>B. subtilis</I> but also by an RNA that is irrelevant to that of <I>B. subtilis</I>. Functional analysis indicated that the growth rate of a Δ<I>ydbR</I> mutant strain of <I>B. subtilis</I> was reduced as compared with that of the wild type not only at 37 °C, but more severely at 22 °C.

収録刊行物

  • Bioscience, biotechnology, and biochemistry  

    Bioscience, biotechnology, and biochemistry 70(7), 1606-1615, 2006-07-23 

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

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各種コード

  • NII論文ID(NAID)
    10018528883
  • NII書誌ID(NCID)
    AA10824164
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09168451
  • NDL 記事登録ID
    7997781
  • NDL 雑誌分類
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z53-G223
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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