A Mucus Adhesion Promoting Protein, MapA, Mediates the Adhesion of Lactobacillus reuteri to Caco-2 Human Intestinal Epithelial Cells
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<I>Lactobacillus reuteri</I> is one of the dominant lactobacilli found in the gastrointestinal tract of various animals. A surface protein of <I>L. reuteri</I> 104R, mucus adhesion promoting protein (MapA), is considered to be an adhesion factor of this strain. We investigated the relation between MapA and adhesion of <I>L. reuteri</I> to human intestinal (Caco-2) cells. Quantitative analysis of the adhesion of <I>L. reuteri</I> strains to Caco-2 cells showed that various <I>L. reuteri</I> strains bind not only to mucus but also to intestinal epithelial cells. In addition, purified MapA bound to Caco-2 cells, and this binding inhibited the adhesion of <I>L. reuteri</I> in a concentration-dependent manner. Based on these observations, the adhesion of <I>L. reuteri</I> appears due to the binding of MapA to receptor-like molecules on Caco-2 cells. Further, far-western analysis indicated the existence of multiple receptor-like molecules in Caco-2 cells.
- Agricultural and Biological Chemistry
Agricultural and Biological Chemistry 70(7), 1622-1628, 2006-07-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry