Purification and Characterization of Cellobiose Dehydrogenase from White-Rot Basidiomycete<i>Trametes hirsuta</i>

• NAKAGAME Seiji

  Technological Initiatives Research Laboratory, Oji Paper Co., Ltd.

• FURUJYO Atsushi

  Technological Initiatives Research Laboratory, Oji Paper Co., Ltd.

• SUGIURA Jun

  Technological Initiatives Research Laboratory, Oji Paper Co., Ltd.

書誌事項

タイトル別名

• Purification and Characterization of Cellobiose Dehydrogenase from White-Rot Basidiomycete Trametes hirsuta

抄録

In order to save energy during the pulp making process, we tried to use white-rot basidiomycete, Trametes hirsuta, which degrades lignin efficiently. But a decrease in paper strength caused by cellulolytic activity ruled this out for practical application. Since the cellulolytic activity of the fungus must be decreased, we purified and characterized a cellobiose dehydrogenase (CDH) that was reported to damage pulp fiber. The CDH in the culture filtrate of C. hirsutus was purified by freeze-thawing and chromatographic methods. The pI of the enzyme was 4.2 and its molecular weight was 92 kDa. The optimal temperature and pH of the enzyme were 60–70 °C and 5.0 respectively. Since the purified CDH decreased the viscosity of pulp in the presence of Fe(III) and cellobiose, it was shown that the suppression of CDH should be an effective way to reduce cellulose damage.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 70 (7), 1629-1635, 2006

  公益社団法人 日本農芸化学会

キーワード

• <I>Trametes hirsuta</I>
• basidiomycete
• cellobiose dehydrogenase

詳細情報

• CRID: 1390001206479858688
• NII論文ID: 10018528962
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.50692
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 7997829
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7997829
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.50692
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可