Expression and Characterization of the Genes Encoding Azoreductases from Bacillus subtilis and Geobacillus stearothermophilus
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Azoreductases have been characterized as enzymes that can decolorize azo dyes by reducing azo groups. In this study, genes encoding proteins having homology with theazoreductase gene of <I>Bacillus</I> sp. OY1-2 were obtained from <I>Bacillus subtilis</I> ATCC6633, <I>B. subtilis</I> ISW1214, and <I>Geobacillus stearotherophilus</I> IFO13737 by polymerase chain reaction. All three genes encoded proteins with 174 amino acids. The deduced amino acid sequences of azoreductase homologs from <I>B. subtilis</I> ISW1214, <I>B. subtilis</I> ATCC6633, and <I>G. stearotherophilus</I> IFO13737 showed similarity of 53.3, 53.9, and 53.3% respectively to that of <I>Bacillus</I> sp. OY1-2.<BR>All three genes were expressed in <I>Escherichia coli</I>, and were characterized as having the decolorizing activity of azo dyes in a β-NADPH dependent manner. The transformation of several azo dyes into colorless compounds by recombinant enzymes was demonstrated to have distinct substrate specificity from that of azoreductase from <I>Bacillus</I> sp. OY1-2.
- Agricultural and Biological Chemistry
Agricultural and Biological Chemistry 70(7), 1655-1665, 2006-07-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry