Expression and Characterization of the Genes Encoding Azoreductases from Bacillus subtilis and Geobacillus stearothermophilus

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Azoreductases have been characterized as enzymes that can decolorize azo dyes by reducing azo groups. In this study, genes encoding proteins having homology with theazoreductase gene of <I>Bacillus</I> sp. OY1-2 were obtained from <I>Bacillus subtilis</I> ATCC6633, <I>B. subtilis</I> ISW1214, and <I>Geobacillus stearotherophilus</I> IFO13737 by polymerase chain reaction. All three genes encoded proteins with 174 amino acids. The deduced amino acid sequences of azoreductase homologs from <I>B. subtilis</I> ISW1214, <I>B. subtilis</I> ATCC6633, and <I>G. stearotherophilus</I> IFO13737 showed similarity of 53.3, 53.9, and 53.3% respectively to that of <I>Bacillus</I> sp. OY1-2.<BR>All three genes were expressed in <I>Escherichia coli</I>, and were characterized as having the decolorizing activity of azo dyes in a β-NADPH dependent manner. The transformation of several azo dyes into colorless compounds by recombinant enzymes was demonstrated to have distinct substrate specificity from that of azoreductase from <I>Bacillus</I> sp. OY1-2.

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  • Bioscience, biotechnology, and biochemistry  

    Bioscience, biotechnology, and biochemistry 70(7), 1655-1665, 2006-07-23 

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

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各種コード

  • NII論文ID(NAID)
    10018529076
  • NII書誌ID(NCID)
    AA10824164
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09168451
  • NDL 記事登録ID
    7997875
  • NDL 雑誌分類
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z53-G223
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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