Expression and Characterization of the Genes Encoding Azoreductases from Bacillus subtilis and Geobacillus stearothermophilus
Azoreductases have been characterized as enzymes that can decolorize azo dyes by reducing azo groups. In this study, genes encoding proteins having homology with theazoreductase gene of <I>Bacillus</I> sp. OY1-2 were obtained from <I>Bacillus subtilis</I> ATCC6633, <I>B. subtilis</I> ISW1214, and <I>Geobacillus stearotherophilus</I> IFO13737 by polymerase chain reaction. All three genes encoded proteins with 174 amino acids. The deduced amino acid sequences of azoreductase homologs from <I>B. subtilis</I> ISW1214, <I>B. subtilis</I> ATCC6633, and <I>G. stearotherophilus</I> IFO13737 showed similarity of 53.3, 53.9, and 53.3% respectively to that of <I>Bacillus</I> sp. OY1-2.<BR>All three genes were expressed in <I>Escherichia coli</I>, and were characterized as having the decolorizing activity of azo dyes in a β-NADPH dependent manner. The transformation of several azo dyes into colorless compounds by recombinant enzymes was demonstrated to have distinct substrate specificity from that of azoreductase from <I>Bacillus</I> sp. OY1-2.
- Bioscience, biotechnology, and biochemistry
Bioscience, biotechnology, and biochemistry 70(7), 1655-1665, 2006-07-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry