Gene Structure and Expression Profile of Cytochrome<i>bc</i>Nitric Oxide Reductase from<i>Hydrogenobacter thermophilus</i>TK-6

• SUZUKI Miho

  Department of Biotechnology, The University of Tokyo

• ARAI Hiroyuki

  Department of Biotechnology, The University of Tokyo

• ISHII Masaharu

  Department of Biotechnology, The University of Tokyo

• IGARASHI Yasuo

  Department of Biotechnology, The University of Tokyo

書誌事項

タイトル別名

• Gene Structure and Expression Profile of Cytochrome bc Nitric Oxide Reductase from Hydrogenobacter thermophilus TK-6

抄録

The genes for a nitric oxide reductase-like cytochrome bc complex were cloned from a thermophilic, chemolithoautotrophic hydrogen-oxidizing bacterium, Hydrogenobacter thermophilus TK-6. The structural genes norC and norB, which encode cytochrome c and cytochrome b subunits of the complex respectively, are probably transcribed as a tricistronic operon with a following gene encoding a putative membrane protein. NorC has, unusually, two hydrophobic transmembrane spans in its N-terminus. Immunoblot analysis showed that expression of NorC was induced by nitrate, nitrite, or sodium nitropurusside, suggesting that the norCB gene product is a denitrification enzyme, nitric oxide reductase. The consensus sequences for the DNR/NnrR-type or the NorR/FhpR-type nitric oxide-sensing regulators of proteobacteria were not found in the norC promoter region, but consensus −35 and −10 sequences were found in this region. These results indicate that strain TK-6 has a nitrogen oxide-sensing regulatory system that differs from proteobacterial systems.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 70 (7), 1666-1671, 2006

  公益社団法人 日本農芸化学会

キーワード

• <I>Hydrogenobacter thermophilus</I>
• nitric oxide reductase
• denitrification
• cytochrome <I>bc</I> complex

詳細情報

• CRID: 1390282681456579712
• NII論文ID: 10018529109
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.60018
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 7997945
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7997945
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.60018
• 本文言語コード: en
• データソース種別:
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  • NDL
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