Gene Structure and Expression Profile of Cytochrome<i>bc</i>Nitric Oxide Reductase from<i>Hydrogenobacter thermophilus</i>TK-6
-
- SUZUKI Miho
- Department of Biotechnology, The University of Tokyo
-
- ARAI Hiroyuki
- Department of Biotechnology, The University of Tokyo
-
- ISHII Masaharu
- Department of Biotechnology, The University of Tokyo
-
- IGARASHI Yasuo
- Department of Biotechnology, The University of Tokyo
書誌事項
- タイトル別名
-
- Gene Structure and Expression Profile of Cytochrome bc Nitric Oxide Reductase from Hydrogenobacter thermophilus TK-6
この論文をさがす
抄録
The genes for a nitric oxide reductase-like cytochrome bc complex were cloned from a thermophilic, chemolithoautotrophic hydrogen-oxidizing bacterium, Hydrogenobacter thermophilus TK-6. The structural genes norC and norB, which encode cytochrome c and cytochrome b subunits of the complex respectively, are probably transcribed as a tricistronic operon with a following gene encoding a putative membrane protein. NorC has, unusually, two hydrophobic transmembrane spans in its N-terminus. Immunoblot analysis showed that expression of NorC was induced by nitrate, nitrite, or sodium nitropurusside, suggesting that the norCB gene product is a denitrification enzyme, nitric oxide reductase. The consensus sequences for the DNR/NnrR-type or the NorR/FhpR-type nitric oxide-sensing regulators of proteobacteria were not found in the norC promoter region, but consensus −35 and −10 sequences were found in this region. These results indicate that strain TK-6 has a nitrogen oxide-sensing regulatory system that differs from proteobacterial systems.
収録刊行物
-
- Bioscience, Biotechnology, and Biochemistry
-
Bioscience, Biotechnology, and Biochemistry 70 (7), 1666-1671, 2006
公益社団法人 日本農芸化学会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390282681456579712
-
- NII論文ID
- 10018529109
-
- NII書誌ID
- AA10824164
-
- ISSN
- 13476947
- 09168451
-
- NDL書誌ID
- 7997945
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
-
- 抄録ライセンスフラグ
- 使用不可