Analysis of the Hyperthermophilic Chitinase from Pyrococcus furiosus : Activity toward Crystalline Chitin

この論文にアクセスする

この論文をさがす

著者

抄録

Chitinase [EC 3.2.1.14] is an enzyme that can hydrolyze the β-1,4 linkage between <I>N</I>-acetyl-<small>D</small>-glucosamine in chitin. In the genome database of the hyperthermophilic archaeon <I>Pyrococcus furiosus</I>, we found two adjacent genes (PF1233 and PF1234) homologous to those of the chitinase of <I>Thermococcus kodakaraensis</I>. In the cultured medium of <I>P. furiosus</I>, however, no chitinase activity was detected. On analysis of the structural gene of <I>P. furiosus</I>, it appears that one nucleotide insertion in PF1234 caused a frame shift and separated a gene. By deletion of one nucleotide in PF1234, the best match was achieved between chitinases of <I>T. kodakaraenesis</I> and <I>P. furiosus</I>. We succeeded in constructing an artificial recombinant chitinase exhibiting hydrolytic activity toward not only colloidal but also crystalline chitins at high temperature. Furthermore, by analyzing the characteristics of the domains, a recombinant enzyme comprising two domains exhibiting high activity toward crystalline chitin was prepared.

収録刊行物

  • Bioscience, biotechnology, and biochemistry  

    Bioscience, biotechnology, and biochemistry 70(7), 1696-1701, 2006-07-23 

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

参考文献:  18件

参考文献を見るにはログインが必要です。ユーザIDをお持ちでない方は新規登録してください。

被引用文献:  1件

被引用文献を見るにはログインが必要です。ユーザIDをお持ちでない方は新規登録してください。

各種コード

  • NII論文ID(NAID)
    10018529263
  • NII書誌ID(NCID)
    AA10824164
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09168451
  • NDL 記事登録ID
    7998005
  • NDL 雑誌分類
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z53-G223
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
ページトップへ