Isolation of Arabidopsis thaliana cDNAs That Confer Yeast Boric Acid Tolerance
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An <I>Arabidopsis thaliana</I> cDNA library was introduced into a <I>Saccharomyces cerevisiae</I> mutant that lacks ScBOR1 (YNL275W), a boron (B) efflux transporter. Five cDNAs were identified that confer tolerance to high boric acid. The nucleotide sequence analysis identified the clones as a polyadenylate-binding protein, AtPAB2; a ribosomal small subunit protein, AtRPS20B; an RNA-binding protein, AtRBP47c′; and two Myb transcription factors, AtMYB13 and AtMYB68. The expression of these five genes also conferred boric acid tolerance on wild-type yeast. Two yeast genes, <I>ScRPS20</I> and <I>ScHRB1</I>, that are similar to the isolated clones, were necessary for this boric acid tolerance. The possible roles of these <I>A. thaliana</I> and <I>S. cerevisiae</I> genes in boric acid tolerance are discussed.
- Agricultural and Biological Chemistry
Agricultural and Biological Chemistry 70(7), 1724-1730, 2006-07-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry