Isolation of<i>Arabidopsis thaliana</i>cDNAs That Confer Yeast Boric Acid Tolerance

• NOZAWA Akira

  Precursory Research for Embryonic Science and Technology Biotechnology Research Center, The University of Tokyo

• MIWA Kyoko

  Biotechnology Research Center, The University of Tokyo

• KOBAYASHI Masaharu

  Biotechnology Research Center, The University of Tokyo

• FUJIWARA Toru

  Precursory Research for Embryonic Science and Technology Biotechnology Research Center, The University of Tokyo

書誌事項

タイトル別名

• Isolation of Arabidopsis thaliana cDNAs That Confer Yeast Boric Acid Tolerance

抄録

An Arabidopsis thaliana cDNA library was introduced into a Saccharomyces cerevisiae mutant that lacks ScBOR1 (YNL275W), a boron (B) efflux transporter. Five cDNAs were identified that confer tolerance to high boric acid. The nucleotide sequence analysis identified the clones as a polyadenylate-binding protein, AtPAB2; a ribosomal small subunit protein, AtRPS20B; an RNA-binding protein, AtRBP47c′; and two Myb transcription factors, AtMYB13 and AtMYB68. The expression of these five genes also conferred boric acid tolerance on wild-type yeast. Two yeast genes, ScRPS20 and ScHRB1, that are similar to the isolated clones, were necessary for this boric acid tolerance. The possible roles of these A. thaliana and S. cerevisiae genes in boric acid tolerance are discussed.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 70 (7), 1724-1730, 2006

  公益社団法人 日本農芸化学会

キーワード

• <I>Arabidopsis thaliana</I>
• boric acid toxicity
• functional complementation
• <I>Saccharomyces cerevisiae</I>

詳細情報

• CRID: 1390282681456585856
• NII論文ID: 10018529370
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.60065
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 7998051
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7998051
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.60065
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可