A Plant Growth Retardant, Uniconazole, Is a Potent Inhibitor of ABA Catabolism in<i>Arabidopsis</i>
-
- SAITO Shigeki
- Institute for Chemical Research, Kyoto University
-
- OKAMOTO Masanori
- Plant Science Center, RIKEN Department of Biological Sciences, Tokyo Metropolitan University
-
- SHINODA Shoko
- Plant Science Center, RIKEN
-
- KUSHIRO Tetsuo
- Plant Science Center, RIKEN
-
- KOSHIBA Tomokazu
- Department of Biological Sciences, Tokyo Metropolitan University
-
- KAMIYA Yuji
- Plant Science Center, RIKEN
-
- HIRAI Nobuhiro
- International Innovation Center, Kyoto University
-
- TODOROKI Yasushi
- Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University
-
- SAKATA Kanzo
- Institute for Chemical Research, Kyoto University
-
- NAMBARA Eiji
- Plant Science Center, RIKEN
-
- MIZUTANI Masaharu
- Institute for Chemical Research, Kyoto University
書誌事項
- タイトル別名
-
- A Plant Growth Retardant, Uniconazole, Is a Potent Inhibitor of ABA Catabolism in Arabidopsis
- Plant Growth Retardant Uniconazole Is a Potent Inhibitor of ABA Catabolism in Arabidopsis
この論文をさがす
抄録
Plant growth retardants (PGRs) reduce the shoot growth of plants by inhibiting gibberellin biosynthesis. In this study, we performed detailed analyses of the inhibitory effects of PGRs on Arabidopsis abscisic acid (ABA) 8′-hydroxylase, a major ABA catabolic enzyme, recently identified as CYP707As. In an in vitro assay with CYP707A3 microsomes expressed in insect cells, uniconazole-P inhibited CYP707A3 activity more effectively than paclobutrazol or tetcyclacis, whereas the other PGRs tested did not inhibit it significantly. Uniconazole-P was found to be a strong competitive inhibitor (Ki=8.0 nM) of ABA 8′-hydroxylase. Uniconazole-P-treated Arabidopsis plants showed enhanced drought tolerance. In uniconazole-P-treated plants, endogenous ABA levels increased 2-fold as compared with the control, and co-application of GA4 did not suppress the effects, indicating that the effects were not due to gibberellin deficiency. Thus uniconazole-P effectively inhibits ABA catabolism in Arabidopsis plants. We also discuss the structure-activity relationship of the azole-type compounds on ABA 8′-hydroxylase inhibitory activity.
収録刊行物
-
- Bioscience, Biotechnology, and Biochemistry
-
Bioscience, Biotechnology, and Biochemistry 70 (7), 1731-1739, 2006
公益社団法人 日本農芸化学会
- Tweet
詳細情報
-
- CRID
- 1390001206479849984
-
- NII論文ID
- 10018529395
-
- NII書誌ID
- AA10824164
-
- ISSN
- 13476947
- 09168451
-
- NDL書誌ID
- 7998063
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可