Cloning and Expression of an α-1,3-Glucanase Gene from Bacillus circulans KA-304 : The Enzyme Participates in Protoplast Formation of Schizophyllum commune

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A culture filtrate of <I>Bacillus circulans</I> KA-304 grown on a cell-wall preparation of <I>Schizophyllum commune</I> has an activity to form protoplasts from <I>S. commune</I> mycelia, and a combination of α-1,3-glucanase and chitinase I, which were isolated from the filtrate, brings about the protoplast-forming activity.<BR>The gene of α-1,3-glucanase was cloned from <I>B. circulans</I> KA-304. It consists of 3,879 nucleotides, which encodes 1,293 amino acids including a putative signal peptide (31 amino acid residues), and the molecular weight of α-1,3-glucanase without the putative signal peptide was calculated to be 132,184. The deduced amino acid sequence of α-1,3-glucanase of <I>B. circulans</I> KA-304 showed approximately 80% similarity to that of mutanase (α-1,3-glucanase) of <I>Bacillus sp.</I> RM1, but no significant similarity to those of fungal mutanases.<BR>The recombinant α-1,3-glucanase was expressed in <I>Escherichia coli</I> Rosetta-gami B (DE 3), and significant α-1,3-glucanase activity was detected in the cell-free extract of the organism treated with isopropyl-β-<small>D</small>-thiogalactopyranoside. The recombinant α-1,3-glucanase showed protoplast-forming activity when the enzyme was combined with chitinase I.

収録刊行物

  • Bioscience, biotechnology, and biochemistry  

    Bioscience, biotechnology, and biochemistry 70(7), 1754-1763, 2006-07-23 

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

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各種コード

  • NII論文ID(NAID)
    10018529474
  • NII書誌ID(NCID)
    AA10824164
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09168451
  • NDL 記事登録ID
    7998107
  • NDL 雑誌分類
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z53-G223
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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