Functional Analysis of an Endo-1,6-β-D-glucanase Gene (neg-1) from Neurospora crassa
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The 1,6-β-<small>D</small>-glucanase gene (<I>neg1</I>) of <I>Neurospora crassa</I> was disrupted by repeat-induced point mutations. Sequence analysis of the <I>neg1</I> gene in the R12-1 mutant showed that 9 nucleotides within the coding region of the gene changed from GC to AT. The base transition of C to A at position 662 resulted in a codon. No apparent phenotypic changes were observed in the mutant, but Congo-red, SDS, and cetyltrimethyl ammonium bromide (CTAB), which affect fungal cell walls or membranes, markedly inhibited the hyphal growth of the mutant at a concentration that does not inhibit growth in the wild type.
- Agricultural and Biological Chemistry
Agricultural and Biological Chemistry 70(7), 1773-1775, 2006-07-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry