Functional Analysis of an Endo-1,6-β-D-glucanase Gene(neg-1) from Neurospora crassa
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- OYAMA Seiji
- Laboratory of Enzymology, Graduate School of Agricultural Science, Tohoku University
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- INOUE Hirokazu
- Laboratory of Genetics, Department of Regulation Biology, Faculty of Science, Saitama University
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- YAMAGATA Youhei
- Laboratory of Enzymology, Graduate School of Agricultural Science, Tohoku University
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- NAKAJIMA Tasuku
- Laboratory of Enzymology, Graduate School of Agricultural Science, Tohoku University
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- ABE Keietu
- Laboratory of Enzymology, Graduate School of Agricultural Science, Tohoku University
書誌事項
- タイトル別名
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- Functional Analysis of an Endo-1,6-.BETA.-D-glucanase Gene (neg-1) from Neurospora crassa
- Functional Analysis of an Endo 1 6 ベータ D glucanase Gene neg 1 from Neurospora crassa
- Functional Analysis of an Endo-1,6-β-<scp>D</scp>-glucanase Gene (<i>neg-1</i>) from<i>Neurospora crassa</i>
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抄録
The 1,6-β-D-glucanase gene (neg1) of Neurospora crassa was disrupted by repeat-induced point mutations. Sequence analysis of the neg1 gene in the R12-1 mutant showed that 9 nucleotides within the coding region of the gene changed from GC to AT. The base transition of C to A at position 662 resulted in a codon. No apparent phenotypic changes were observed in the mutant, but Congo-red, SDS, and cetyltrimethyl ammonium bromide (CTAB), which affect fungal cell walls or membranes, markedly inhibited the hyphal growth of the mutant at a concentration that does not inhibit growth in the wild type.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 70 (7), 1773-1775, 2006
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206479831424
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- NII論文ID
- 10018529550
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 7998170
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- 抄録ライセンスフラグ
- 使用不可