Properties of a Trifluoroleucine-Resistant Mutant of Saccharomyces cerevisiae
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We characterized a trifluoroleucine-resistant mutant of <I>Saccharomyces cerevisiae</I>, TFL20, that has a mutation in the <I>LEU4</I> gene. We monitored the concentration of extracellular <I>i</I>-AmOH and intracellular amino acids, and compared the ratios of gene expression in TFL20 with the wild-type strain, K30. We found that the <I>LEU1</I>, <I>LEU2</I>, and <I>BAT1</I> genes were up-regulated in TFL20 for metabolism, and that TFL20 simultaneously produced as much <I>i</I>-AmOH and leucine as K30 does.
- Agricultural and Biological Chemistry
Agricultural and Biological Chemistry 70(7), 1776-1779, 2006-07-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry