Properties of a Trifluoroleucine-Resistant Mutant of Saccharomyces cerevisiae

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  • OBA Takahiro
    Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center
  • YAMAMOTO Yoshitsugu
    Graduate School of Genetic Resources Technology, Kyushu University
  • NOMIYAMA Shuji
    Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center
  • SUENAGA Hikaru
    Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center
  • MUTA Shigeru
    Graduate School of Genetic Resources Technology, Kyushu University
  • TASHIRO Kosuke
    Graduate School of Genetic Resources Technology, Kyushu University
  • KUHARA Satoru
    Graduate School of Genetic Resources Technology, Kyushu University

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  • Properties of a Trifluoroleucine-Resistant Mutant of<i>Saccharomyces cerevisiae</i>

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Abstract

We characterized a trifluoroleucine-resistant mutant of Saccharomyces cerevisiae, TFL20, that has a mutation in the LEU4 gene. We monitored the concentration of extracellular i-AmOH and intracellular amino acids, and compared the ratios of gene expression in TFL20 with the wild-type strain, K30. We found that the LEU1, LEU2, and BAT1 genes were up-regulated in TFL20 for metabolism, and that TFL20 simultaneously produced as much i-AmOH and leucine as K30 does.

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