Simple Identification of Transgenic Arabidopsis Plants Carrying a Single Copy of the Integrated Gene
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Transgenic <I>Arabidopsis thaliana</I> plants carrying a single copy of integrated DNA can be identified by single-step genomic polymerase chain reaction. The reaction employs two sets of primer pairs with the same melting temperature that amplify the amplicons derived from the integrated T-DNA together with those from an endogenous single-copy gene as a reference. When the band intensity ratio is one, this means that the transgenic plants are carrying a single copy of the integrated gene per haploid.
- Agricultural and Biological Chemistry
Agricultural and Biological Chemistry 70(7), 1780-1783, 2006-07-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry