Genetically Engineered Poly-γ-glutamate Producer from Bacillus subtilis ISW1214
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The <I>pgsBCA</I>-gene disruptant from <I>Bacillus subtilis</I> ISW1214, <I>i.e.</I>, MA41, does not produce poly-γ-glutamate (PGA). We newly constructed an MA41 recombinant bearing the plasmid-borne PGA synthetic system, in which PGA production was strictly controlled by the use of xylose. Unlike the parent strain, ISW1214, the genetically engineered strain produced abundant PGA in both <small>L</small>-glutamate-rich and <small>D</small>-glutamate-rich media.
- Agricultural and Biological Chemistry
Agricultural and Biological Chemistry 70(7), 1794-1797, 2006-07-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry