Intron-Dependent Accumulation of mRNA in Coriolus hirsutus of Lignin Peroxidase Gene the Product of Which Is Involved in Conversion/Degradation of Polychlorinated Aromatic Hydrocarbons
The homobasidiomycete <I>Coriolus hirsutus</I> coding sequences of a lignin peroxidase (LiP) gene (<I>lip</I>, containing six (I–VI) introns), a <I>lip</I> cDNA (<I>lip</I>c), and three <I>lip</I>c derivatives containing one (I), three (I–III), or five (I–V) introns were inserted into chromosome-integrating expression vector. These recombinant plasmids were introduced into <I>C. hirustus</I> monokaryotic strain. The transformant carrying the promoter–<I>lip</I>c–terminator cassette did not contain enough mRNA molecules to be detectable by Northern-blot analysis. On the other hand, all the transformants carrying cassettes of genomic <I>lip</I> and intron(s)-containing <I>lip</I>c sequences contained sufficient amounts of mRNAs to be easily detected by Northern-blot analysis. LiP activities in the culture supernatants of these transformants were found to be about five times as high as those of transformants carrying the <I>lip</I>c cassette (or no cassette). The culture supernatants of the transformants with high LiP activity showed remarkably high conversion activity toward pentachlorophenol (PCP) and degradation activity toward 2,7-dichlorodibenzo-<I>p</I>-dioxin (2,7-DCDD). These results indicate that at least one intron (intron I) is required for accumulation of <I>lip</I> mRNA and its subsequent translational expression in <I>C. hirsutus</I>.
- Bioscience, biotechnology, and biochemistry
Bioscience, biotechnology, and biochemistry 70(6), 1293-1299, 2006-06-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry