Intron-Dependent Accumulation of mRNA in Coriolus hirsutus of Lignin Peroxidase Gene the Product of Which Is Involved in Conversion/Degradation of Polychlorinated Aromatic Hydrocarbons

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The homobasidiomycete <I>Coriolus hirsutus</I> coding sequences of a lignin peroxidase (LiP) gene (<I>lip</I>, containing six (I–VI) introns), a <I>lip</I> cDNA (<I>lip</I>c), and three <I>lip</I>c derivatives containing one (I), three (I–III), or five (I–V) introns were inserted into chromosome-integrating expression vector. These recombinant plasmids were introduced into <I>C. hirustus</I> monokaryotic strain. The transformant carrying the promoter–<I>lip</I>c–terminator cassette did not contain enough mRNA molecules to be detectable by Northern-blot analysis. On the other hand, all the transformants carrying cassettes of genomic <I>lip</I> and intron(s)-containing <I>lip</I>c sequences contained sufficient amounts of mRNAs to be easily detected by Northern-blot analysis. LiP activities in the culture supernatants of these transformants were found to be about five times as high as those of transformants carrying the <I>lip</I>c cassette (or no cassette). The culture supernatants of the transformants with high LiP activity showed remarkably high conversion activity toward pentachlorophenol (PCP) and degradation activity toward 2,7-dichlorodibenzo-<I>p</I>-dioxin (2,7-DCDD). These results indicate that at least one intron (intron I) is required for accumulation of <I>lip</I> mRNA and its subsequent translational expression in <I>C. hirsutus</I>.

収録刊行物

  • Bioscience, biotechnology, and biochemistry  

    Bioscience, biotechnology, and biochemistry 70(6), 1293-1299, 2006-06-23 

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

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各種コード

  • NII論文ID(NAID)
    10018529724
  • NII書誌ID(NCID)
    AA10824164
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09168451
  • NDL 記事登録ID
    7958700
  • NDL 雑誌分類
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z53-G223
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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