Characterization of Sulfolobus solfataricus 2-Keto-3-deoxy-D-gluconate Kinase in the Modified Entner-Doudoroff Pathway

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The thermoacidophilic archaeon <I>Sulfolobus solfataricus</I> is known to utilize <small>D</small>-glucose <I>via</I> the nonphosphorylated Entner-Doudoroff (ED) pathway. But, the genome database shows that this microorganism has a gene (<I>kdgK</I>) encoding 2-keto-3-deoxy-<small>D</small>-gluconate (KDG) kinase (KDGK) which phosphorylates KDG to 2-keto-3-deoxy-6-phosphogluconate. Interestingly, <I>kdgK</I> and three other genes in the modified ED pathway are organized as an operon-like structure. In this study, we report confirmation of the catalytic activity of the <I>S. solfataricus</I> KDGK protein. We also found that the <I>kdgK</I> gene was transcribed as polycistronic transcripts. Proteome analysis of cell lysate revealed that all gene products in the <I>kdgK</I> operon were expressed as functional proteins. These results strongly indicate that <I>S. solfataricus</I> metabolizes <small>D</small>-glucose <I>via</I> the 'partially' nonphosphorylated ED pathway. A purified recombinant <I>S. solfataricus</I> KDGK had <I>K</I><SUB>m</SUB> and <I>k</I><SUB>cat</SUB> values of 0.14 m<small>M</small> and 60.8 s<SUP>−1</SUP> respectively for KDG, and showed maximal activity at temperatures between 70 and 80 °C and pHs between 7.0 and 8.0.

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  • Bioscience, biotechnology, and biochemistry  

    Bioscience, biotechnology, and biochemistry 70(6), 1308-1316, 2006-06-23 

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

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各種コード

  • NII論文ID(NAID)
    10018529786
  • NII書誌ID(NCID)
    AA10824164
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09168451
  • NDL 記事登録ID
    7958742
  • NDL 雑誌分類
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z53-G223
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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