Glycoform Analysis of N-Glycans Linked to Glycoproteins Expressed in Rice Culture Cells : Predominant Occurrence of Complex Type N-Glycans

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著者

    • Maeda Megumi MAEDA Megumi
    • Division of Bioscience, Department of Biofunctional Chemistry, Graduate School of Natural Science and Technology, Okayama University

抄録

Although it has been found that plant endo-β-<I>N</I>-acetylglucosaminidase shows strong activity towards denatured glycoproteins and glycopeptides with high-mannose type <I>N</I>-glycans and free high-mannose type <I>N</I>-glycans bearing the chitobiosyl unit, the endogenous substrates for plant endoglycosidase have not yet been identified. Recently we purified and characterized an endo-β-<I>N</I>-acetylglucosaminidase from rice culture cells and identified the gene encoded (Maeda, M., and Kimura, Y., <I>Trends Glycosci. Glycotech.</I>, <B>17</B>, 205–214 (2005)). Furthermore, we found structural features of free <I>N</I>-glycans in the cells, indicating that high-mannose type species (Man<SUB>9-5</SUB>GlcNAc<SUB>1</SUB>) occur at concentration of several micromolar (μ<small>M</small>). Hence, in this study we analyzed glycoform of <I>N</I>-glycans linked to glycoproteins expressed in rice culture cells to see whether endogenous glycoproteinous substrate occurs in reasonable amounts. Structural analysis revealed that more than 95% of total <I>N</I>-glycans linked to glycoproteins in the rice cells had the plant complex type structure, including Lewis a epitope-harboring type, although high-mannose type structures account for less than 5% of total <I>N</I>-glycans.

収録刊行物

  • Bioscience, biotechnology, and biochemistry  

    Bioscience, biotechnology, and biochemistry 70(6), 1356-1363, 2006-06-23 

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

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各種コード

  • NII論文ID(NAID)
    10018529941
  • NII書誌ID(NCID)
    AA10824164
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09168451
  • NDL 記事登録ID
    7958826
  • NDL 雑誌分類
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z53-G223
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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