Production of α,ω-Alkanediols Using Escherichia coli Expressing a Cytochrome P450 from Acinetobacter sp. OC4
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Our biotransformation using <I>Escherichia coli</I> expressing a cytochrome P450 (CYP) belonging to the CYP153A family from <I>Acinetobacter</I> sp. OC4 produced a great amount of 1-octanol (2,250 mg per liter) from <I>n</I>-octane after 24 h of incubation. This level of production is equivalent to the maximum level previously achieved in biotransformation experiments of alkanes. In addition, the initial production rate of 1-octanol was maintained throughout the entire incubation period. These results indicate that we have achieved the functional and stable expression of a CYP in <I>E. coli</I> for the first time. Further, our biotransformation system showed α,ω-diterminal oxidation activity of <I>n</I>-alkanes, and a large amount of 1,8-octanediol (722 mg per liter) was produced from 1-octanol after 24 h of incubation. This is the first report on the bioproduction of α,ω-alkanediols from <I>n</I>-alkanes or 1-alkanols.
- Agricultural and Biological Chemistry
Agricultural and Biological Chemistry 70(6), 1379-1385, 2006-06-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry