Production of α,ω-Alkanediols Using Escherichia coli Expressing a Cytochrome P450 from Acinetobacter sp. OC4

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  • Production of .ALPHA.,.OMEGA.-Alkanediols Using Escherichia coli Expressing a Cytochrome P450 from Acinetobacter sp. OC4
  • Production of アルファ オメガ Alkanediols Using Escherichia coli Expressing a Cytochrome P450 from Acinetobacter sp OC4
  • Production of α,ω-Alkanediols Using<i>Escherichia coli</i>Expressing a Cytochrome P450 from<i>Acinetobacter</i>sp. OC4

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Our biotransformation using Escherichia coli expressing a cytochrome P450 (CYP) belonging to the CYP153A family from Acinetobacter sp. OC4 produced a great amount of 1-octanol (2,250 mg per liter) from n-octane after 24 h of incubation. This level of production is equivalent to the maximum level previously achieved in biotransformation experiments of alkanes. In addition, the initial production rate of 1-octanol was maintained throughout the entire incubation period. These results indicate that we have achieved the functional and stable expression of a CYP in E. coli for the first time. Further, our biotransformation system showed α,ω-diterminal oxidation activity of n-alkanes, and a large amount of 1,8-octanediol (722 mg per liter) was produced from 1-octanol after 24 h of incubation. This is the first report on the bioproduction of α,ω-alkanediols from n-alkanes or 1-alkanols.

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