Characterization of Flavonoid 7-O-Glucosyltransferase from Arabidopsis thaliana

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著者

    • KO Jae Hyung
    • Bio/Molecular Informatics Center, Division of Bioscience and Biotechnology, Konkuk University
    • LIM Chae Eun
    • Bio/Molecular Informatics Center, Division of Bioscience and Biotechnology, Konkuk University
    • LIM Jun
    • Bio/Molecular Informatics Center, Division of Bioscience and Biotechnology, Konkuk University
    • LIM Yoongho
    • Bio/Molecular Informatics Center, Division of Bioscience and Biotechnology, Konkuk University
    • AHN Joong-Hoon
    • Bio/Molecular Informatics Center, Division of Bioscience and Biotechnology, Konkuk University

抄録

Most flavonoids found in plants exist as glycosides, and glycosylation status has a wide range of effects on flavonoid solubility, stability, and bioavailability. Glycosylation of flavonoids is mediated by Family 1 glycosyltransferases (UGTs), which use UDP-sugars, such as UDP-glucose, as the glycosyl donor. <I>AtGT-2</I>, a UGT from <I>Arabidopsis thaliana</I>, was cloned and expressed in <I>Escherichia coli</I> as a gluthatione <I>S</I>-transferase fusion protein. Several compounds, including flavonoids, were tested as potential substrates. HPLC analysis of the reaction products indicated that AtGT-2 transfers a glucose molecule into several different kinds of flavonoids, eriodictyol being the most effective substrate, followed by luteolin, kaempferol, and quercetin. Based on comparison of HPLC retention times with authentic flavonoid 7-<I>O</I>-glucosides and nuclear magnetic resonance spectroscopy, the glycosylation position in the reacted flavonoids was determined to be the C-7 hydroxyl group. These results indicate that <I>AtGT-2</I> encodes a flavonoid 7-<I>O</I>-glucosyltransferase.

収録刊行物

  • Bioscience, biotechnology, and biochemistry  

    Bioscience, biotechnology, and biochemistry 70(6), 1471-1477, 2006-06-23 

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

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各種コード

  • NII論文ID(NAID)
    10018530372
  • NII書誌ID(NCID)
    AA10824164
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09168451
  • NDL 記事登録ID
    7959006
  • NDL 雑誌分類
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z53-G223
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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