Characterization of Arylsulfatase Formed by Derepressed Synthesis in Citrobacter braakii
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Arylsulfatase activity was detected in a bacterial strain, <I>Citrobacter braakii</I> 69-b, isolated from soil by enrichment cultivation using porcine gastric mucin. The production of arylsulfatase was derepressed markedly in a synthetic medium by the addition of tyramine. The purified enzyme hydrolyzed 4-nitrophenyl sulfate, 4-nitrocatechol sulfate, and 3-indoxyl sulfate, and was classified as type I arylsulfatase.
- Agricultural and Biological Chemistry
Agricultural and Biological Chemistry 70(6), 1509-1512, 2006-06-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry