The Effect of Pyruvate Decarboxylase Gene Knockout in Saccharomyces cerevisiae on L-Lactic Acid Production

• ISHIDA Nobuhiro

  Biotechnology Laboratory, Toyota Central R&D Labs Inc.

• SAITOH Satoshi

  Toyota Biotechnology & Afforestation Laboratory, Toyota Motor Co.

• ONISHI Toru

  Toyota Biotechnology & Afforestation Laboratory, Toyota Motor Co.

• TOKUHIRO Kenro

  Biotechnology Laboratory, Toyota Central R&D Labs Inc.

• NAGAMORI Eiji

  Biotechnology Laboratory, Toyota Central R&D Labs Inc.

• KITAMOTO Katsuhiko

  Department of Biotechnology, The University of Tokyo

• TAKAHASHI Haruo

  Biotechnology Laboratory, Toyota Central R&D Labs Inc.

Bibliographic Information

Other Title

• Effect of Pyruvate Decarboxylase Gene Knockout in Saccharomyces cerevisiae on L Lactic Acid Production
• The Effect of Pyruvate Decarboxylase Gene Knockout in<i>Saccharomyces cerevisiae</i>on<scp>L</scp>-Lactic Acid Production

Abstract

A plant- and crop-based renewable plastic, poly-lactic acid (PLA), is receiving attention as a new material for a sustainable society in place of petroleum-based plastics. We constructed a metabolically engineered Saccharomyces cerevisiae that has both pyruvate decarboxylase genes (PDC1 and PDC5) disrupted in the genetic background to express two copies of the bovine L-lactate dehydrogenase (LDH) gene. With this recombinant, the yield of lactate was 82.3 g/liter, up to 81.5% of the glucose being transformed into lactic acid on neutralizing cultivation, although pdc1 pdc5 double disruption led to ineffective decreases in cell growth and fermentation speed. This strain showed lactate productivity improvement as much as 1.5 times higher than the previous strain. This production yield is the highest value for a lactic acid-producing yeast yet reported.

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 70 (5), 1148-1153, 2006

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• lactic acid production
• <I>Saccharomyces cerevisiae</I>
• pyruvate decarboxylase
• genome integration

Details

• CRID: 1390282681449510656
• NII Article ID: 10018531187
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.70.1148
• ISSN: 13476947
• ISSN: 09168451
• NDL BIB ID: 7926127
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7926127
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.70.1148
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed