Improvement of the glaB Promoter Expressed in Solid-State Fermentation (SSF) of Aspergillus oryzae
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The glucoamylase-encoding gene (<I>glaB</I>) promoter should be very useful for recombinant protein production in solid-state fermentation (SSF) of <I>Aspergillus oryzae</I>. A 97-bp fragment containing the <I>cis</I>-element of the <I>glaB</I> promoter was inserted into the <I>glaA</I> promoter, which was little expressed in SSF. The chimeric promoter showed about a 24-fold increase in promoter activity in SSF. Eight copies of the 97-bp fragment were tandemly fused with the <I>glaB</I> promoter. The improved promoter showed about a 4.6-fold increase in promoter activity in SSF. The <I>glaB</I> gene was overexpressed under control of the improved <I>glaB</I> promoter in SSF. Recombinant glucoamylase production reached about 1524 mg/kg-broth for 2 d. The improved <I>glaB</I> promoter should be very useful for overproduction of a recombinant protein in SSF of <I>A. oryzae</I>.
- Agricultural and Biological Chemistry
Agricultural and Biological Chemistry 70(5), 1181-1187, 2006-05-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry