Improvement of the glaB Promoter Expressed in Solid-State Fermentation (SSF) of Aspergillus oryzae
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- ISHIDA Hiroki
- Research Institute, Gekkeikan Sake Co., Ltd.
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- HATA Yoji
- Research Institute, Gekkeikan Sake Co., Ltd.
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- KAWATO Akitsugu
- Research Institute, Gekkeikan Sake Co., Ltd.
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- ABE Yasuhisa
- Research Institute, Gekkeikan Sake Co., Ltd.
Bibliographic Information
- Other Title
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- Improvement of the<i>glaB</i>Promoter Expressed in Solid-State Fermentation (SSF) of<i>Aspergillus oryzae</i>
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Abstract
The glucoamylase-encoding gene (glaB) promoter should be very useful for recombinant protein production in solid-state fermentation (SSF) of Aspergillus oryzae. A 97-bp fragment containing the cis-element of the glaB promoter was inserted into the glaA promoter, which was little expressed in SSF. The chimeric promoter showed about a 24-fold increase in promoter activity in SSF. Eight copies of the 97-bp fragment were tandemly fused with the glaB promoter. The improved promoter showed about a 4.6-fold increase in promoter activity in SSF. The glaB gene was overexpressed under control of the improved glaB promoter in SSF. Recombinant glucoamylase production reached about 1524 mg/kg-broth for 2 d. The improved glaB promoter should be very useful for overproduction of a recombinant protein in SSF of A. oryzae.
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 70 (5), 1181-1187, 2006
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Keywords
Details 詳細情報について
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- CRID
- 1390001206477060992
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- NII Article ID
- 10018531307
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- NII Book ID
- AA10824164
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- COI
- 1:CAS:528:DC%2BD28XlvVCjsLg%3D
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 7926268
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- PubMed
- 16717420
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed