Identification of 2-(Cysteinyl)amido-2-deoxy-D-galacturonic Acid Residue from the Sheath of Leptothrix cholodnii

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著者

    • MIYANOIRI Yohei
    • Department of Supramolecular Biology, International Graduate School of Arts and Sciences, Yokohama City University
    • KATAHIRA Masato
    • Department of Supramolecular Biology, International Graduate School of Arts and Sciences, Yokohama City University
    • TAKEDA Minoru
    • Division of Materials Science and Chemical Engineering, Faculty of Engineering, Yokonama National University
    • KOIZUMI Jun-ichi
    • Division of Materials Science and Chemical Engineering, Faculty of Engineering, Yokonama National University

抄録

The sheath of <I>Leptothrix cholodnii</I> is a glycoconjugate composed of a polysaccharide and a peptide rich in cysteine. In this study, structural determination of the hydrazinolyzate of the sheath was carried out. Since the hydrazinolyzate is a polysaccharide incorporated with cysteine, it was <I>S</I>-derivatized with a thiol-specific fluorogenic reagent, 4-(aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole (ABD-F). Fluorescent fragments were purified by HPLC, and their structures were analyzed by mass spectrometry and NMR spectroscopy. The sheath was found to contain 2-(cysteinyl)amido-2-deoxy-<small>D</small>-galacturonic acid residue.

収録刊行物

  • Bioscience, biotechnology, and biochemistry  

    Bioscience, biotechnology, and biochemistry 70(5), 1265-1268, 2006-05-23 

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

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各種コード

  • NII論文ID(NAID)
    10018531700
  • NII書誌ID(NCID)
    AA10824164
  • 本文言語コード
    ENG
  • 資料種別
    NOT
  • ISSN
    09168451
  • NDL 記事登録ID
    7926566
  • NDL 雑誌分類
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z53-G223
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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