Identification of 2-(Cysteinyl)amido-2-deoxy-D-galacturonic Acid Residue from the Sheath of Leptothrix cholodnii
The sheath of <I>Leptothrix cholodnii</I> is a glycoconjugate composed of a polysaccharide and a peptide rich in cysteine. In this study, structural determination of the hydrazinolyzate of the sheath was carried out. Since the hydrazinolyzate is a polysaccharide incorporated with cysteine, it was <I>S</I>-derivatized with a thiol-specific fluorogenic reagent, 4-(aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole (ABD-F). Fluorescent fragments were purified by HPLC, and their structures were analyzed by mass spectrometry and NMR spectroscopy. The sheath was found to contain 2-(cysteinyl)amido-2-deoxy-<small>D</small>-galacturonic acid residue.
- Bioscience, biotechnology, and biochemistry
Bioscience, biotechnology, and biochemistry 70(5), 1265-1268, 2006-05-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry