Identification of 2-(Cysteinyl)amido-2-deoxy-D-galacturonic Acid Residue from the Sheath of Leptothrix cholodnii

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  • MAKITA Hiroko
    Division of Materials Science and Chemical Engineering, Faculty of Engineering, Yokonama National University
  • NAKAHARA Yuichi
    Division of Materials Science and Chemical Engineering, Faculty of Engineering, Yokonama National University
  • FUKUI Hiroyuki
    Division of Materials Science and Chemical Engineering, Faculty of Engineering, Yokonama National University
  • MIYANOIRI Yohei
    Department of Supramolecular Biology, International Graduate School of Arts and Sciences, Yokohama City University
  • KATAHIRA Masato
    Department of Supramolecular Biology, International Graduate School of Arts and Sciences, Yokohama City University Genome Science Center, RIKEN PRESTO, JST
  • SEKI Hiroko
    Chemical Analysis Center, Chiba University
  • TAKEDA Minoru
    Division of Materials Science and Chemical Engineering, Faculty of Engineering, Yokonama National University
  • KOIZUMI Jun-ichi
    Division of Materials Science and Chemical Engineering, Faculty of Engineering, Yokonama National University

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  • Identification of 2-(Cysteinyl)amido-2-deoxy-<scp>D</scp>-galacturonic Acid Residue from the Sheath of<i>Leptothrix cholodnii</i>

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Abstract

The sheath of Leptothrix cholodnii is a glycoconjugate composed of a polysaccharide and a peptide rich in cysteine. In this study, structural determination of the hydrazinolyzate of the sheath was carried out. Since the hydrazinolyzate is a polysaccharide incorporated with cysteine, it was S-derivatized with a thiol-specific fluorogenic reagent, 4-(aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole (ABD-F). Fluorescent fragments were purified by HPLC, and their structures were analyzed by mass spectrometry and NMR spectroscopy. The sheath was found to contain 2-(cysteinyl)amido-2-deoxy-D-galacturonic acid residue.

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