Production of Three O-Methhylated Esculetins with Escherichia coli Expressing O-Methyltransferase from Poplar

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著者

    • Hur Hor-Gil [他] HUR Hor-Gil
    • Department of Environmental Science and Engineering and International Environmental Research Center, Gwangju Institute of Science and Technology
    • LIM Yoongho
    • Bio/Molecular Informatics Center, Department of Bioscience and Biotechnology, Konkuk University
    • AHN Joong-Hoon
    • Bio/Molecular Informatics Center, Department of Bioscience and Biotechnology, Konkuk University

抄録

<I>O</I>-Methyltransferase, POMT-9 was expressed in <I>Escherichia coli</I>. HPLC analysis of reaction products revealed three peaks corresponding to isoscopoletin, scopoletin, and scoparone, and their structures were determined using NMR. Biotransformation of esculetin with <I>E. coli</I> expressing POMT-9 generated scopoletin, isoscopoletin, and scoparone at 30.3, 21, and 31 μ<small>M</small> respectively. POMT-9 is the first <I>O</I>-methyltransferase that produces three different <I>O</I>-methylated products.

収録刊行物

  • Bioscience, biotechnology, and biochemistry  

    Bioscience, biotechnology, and biochemistry 70(5), 1269-1272, 2006-05-23 

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

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各種コード

  • NII論文ID(NAID)
    10018531713
  • NII書誌ID(NCID)
    AA10824164
  • 本文言語コード
    ENG
  • 資料種別
    NOT
  • ISSN
    09168451
  • NDL 記事登録ID
    7926587
  • NDL 雑誌分類
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z53-G223
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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