Production of Three<i>O</i>-Methhylated Esculetins with<i>Escherichia coli</i>Expressing<i>O</i>-Methyltransferase from Poplar
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- KIM Bong Gyu
- Bio/Molecular Informatics Center, Department of Bioscience and Biotechnology, Konkuk University
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- LEE Youngshim
- Bio/Molecular Informatics Center, Department of Bioscience and Biotechnology, Konkuk University
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- HUR Hor-Gil
- Department of Environmental Science and Engineering and International Environmental Research Center, Gwangju Institute of Science and Technology
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- LIM Yoongho
- Bio/Molecular Informatics Center, Department of Bioscience and Biotechnology, Konkuk University
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- AHN Joong-Hoon
- Bio/Molecular Informatics Center, Department of Bioscience and Biotechnology, Konkuk University
書誌事項
- タイトル別名
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- Production of Three O-Methhylated Esculetins with Escherichia coli Expressing O-Methyltransferase from Poplar
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抄録
O-Methyltransferase, POMT-9 was expressed in Escherichia coli. HPLC analysis of reaction products revealed three peaks corresponding to isoscopoletin, scopoletin, and scoparone, and their structures were determined using NMR. Biotransformation of esculetin with E. coli expressing POMT-9 generated scopoletin, isoscopoletin, and scoparone at 30.3, 21, and 31 μM respectively. POMT-9 is the first O-methyltransferase that produces three different O-methylated products.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 70 (5), 1269-1272, 2006
公益社団法人 日本農芸化学会
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詳細情報
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- CRID
- 1390001206476999936
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- NII論文ID
- 10018531713
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 7926587
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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