Production of Three O-Methhylated Esculetins with Escherichia coli Expressing O-Methyltransferase from Poplar
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<I>O</I>-Methyltransferase, POMT-9 was expressed in <I>Escherichia coli</I>. HPLC analysis of reaction products revealed three peaks corresponding to isoscopoletin, scopoletin, and scoparone, and their structures were determined using NMR. Biotransformation of esculetin with <I>E. coli</I> expressing POMT-9 generated scopoletin, isoscopoletin, and scoparone at 30.3, 21, and 31 μ<small>M</small> respectively. POMT-9 is the first <I>O</I>-methyltransferase that produces three different <I>O</I>-methylated products.
- Agricultural and Biological Chemistry
Agricultural and Biological Chemistry 70(5), 1269-1272, 2006-05-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry