Production of Three O-Methhylated Esculetins with Escherichia coli Expressing O-Methyltransferase from Poplar
<I>O</I>-Methyltransferase, POMT-9 was expressed in <I>Escherichia coli</I>. HPLC analysis of reaction products revealed three peaks corresponding to isoscopoletin, scopoletin, and scoparone, and their structures were determined using NMR. Biotransformation of esculetin with <I>E. coli</I> expressing POMT-9 generated scopoletin, isoscopoletin, and scoparone at 30.3, 21, and 31 μ<small>M</small> respectively. POMT-9 is the first <I>O</I>-methyltransferase that produces three different <I>O</I>-methylated products.
- Bioscience, biotechnology, and biochemistry
Bioscience, biotechnology, and biochemistry 70(5), 1269-1272, 2006-05-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry