Quantification of Genetically Modified Soybeans Using a Combination of a Capillary-Type Real-Time PCR System and a Plasmid Reference Standard

• TOYOTA Akie

  Hiroshima Prefectural Institute of Public Health and Environment Graduate School of Biosphere Sciences, Hiroshima University

• AKIYAMA Hiroshi

  National Institute of Health Sciences

• SUGIMURA Mitsunori

  Hiroshima Prefectural Institute of Public Health and Environment

• WATANABE Takahiro

  National Institute of Health Sciences

• KIKUCHI Hiroyuki

  National Institute of Health Sciences

• KANAMORI Hisayuki

  Hiroshima Prefectural Institute of Public Health and Environment

• HINO Akihiro

  National Food Research Institute

• ESAKA Muneharu

  Graduate School of Biosphere Sciences, Hiroshima University

• MAITANI Tamio

  National Institute of Health Sciences

Abstract

Because the labeling of grains and feed- and foodstuffs is mandatory if the genetically modified organism (GMO) content exceeds a certain level of approved genetically modified varieties in many countries, there is a need for a rapid and useful method of GMO quantification in food samples. In this study, a rapid detection system was developed for Roundup Ready^® Soybean (RRS) quantification using a combination of a capillary-type real-time PCR system, a LightCycler^® real-time PCR system, and plasmid DNA as the reference standard. In addition, we showed for the first time that the plasmid and genomic DNA should be similar in the established detection system because the PCR efficiencies of using plasmid DNA and using genomic DNA were not significantly different. The conversion factor (C_f) to calculate RRS content (%) was further determined from the average value analyzed in three laboratories. The accuracy and reproducibility of this system for RRS quantification at a level of 5.0% were within a range from 4.46 to 5.07% for RRS content and within a range from 2.0% to 7.0% for the relative standard deviation (RSD) value, respectively. This system rapidly monitored the labeling system and had allowable levels of accuracy and precision.

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 70 (4), 821-827, 2006

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• genetically modified soybean
• Roundup Ready<SUP>®</SUP> Soybean
• capillary-type real-time PCR system

Details

• CRID: 1390001206476586880
• NII Article ID: 10018532042
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.70.821
• ISSN: 13476947
• ISSN: 09168451
• NDL BIB ID: 7899603
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7899603
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.70.821
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed