Isolation and Characteristics of Methanosaeta in Paddy Field Soils
The population of filamentous acetate-utilizing methanogens in paddy field soils was 2.0×10<SUP>4</SUP> MPN/g dry soil in the submerged condition. They were able to form colonies in a deep agar medium, but not in a roll tube. Filamentous acetate-utilizing methanogens isolated from Kanagi, Japan (strain K-5) and Tsukuba, Japan (strain T-3) were divided into two types based on length of filaments. One type, strain K-5, formed a short chain which was dispersed easily by weak shaking. The other type, strain T-3, formed a long chain, which formed cotton-like flocs and was not dispersed by weak shaking. They had sheaths composed of a pair of adjacent membranes on the outside of the cell membranes. The 16S rRNA gene similarities of strain T-3 and K-5 to <I>Methanosaeta concilii</I> strain Opfikon were 100% and 99.5% respectively. Filamentous acetate-utilizing methanogens were also isolated from paddy field soils in various other regions of Japan. Our results suggest that <I>Methanosaeta</I> is universal in paddy soils and that it plays an important role in methane production from acetate.
- Bioscience, biotechnology, and biochemistry
Bioscience, biotechnology, and biochemistry 70(4), 828-835, 2006-04-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry