Enzymatic Production of Highly Soluble Myricitrin Glycosides Using β-Galactosidase

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Myricitrin, a botanical flavonol glycoside, could be a useful ingredient of functional foods, cosmetics, and medicines because of its high anti-oxidative activity. However, due to its insolubility in water, it has a limited range of use. To improve this solubility, we glycosylated myricitrin by an enzymatic transglycosylate reaction. Myricitrin was galactosylated by β-galactosidase from <I>Bacillus circulans</I> using lactose as a sugar donor. The reaction product was 480 times more soluble than myricitrin. Four myricitrin galactosides were isolated from the reaction products by column chromatography, and their molecular structures were identified by using ESI-MS, <SUP>1</SUP>H-NMR, <SUP>13</SUP>C-NMR, <SUP>1</SUP>H–<SUP>1</SUP>H COSY, <SUP>1</SUP>H-<SUP>13</SUP>C HMQC and <SUP>1</SUP>H-<SUP>13</SUP>C HMBC analysis. The solubility of these four myricitrin galactosides was more than 3.9×10<SUP>3</SUP> fold that of myricitrin, and each had similar anti-oxidative activity to that of myricitrin.

収録刊行物

  • Bioscience, biotechnology, and biochemistry  

    Bioscience, biotechnology, and biochemistry 70(4), 940-948, 2006-04-23 

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

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各種コード

  • NII論文ID(NAID)
    10018532524
  • NII書誌ID(NCID)
    AA10824164
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09168451
  • NDL 記事登録ID
    7899900
  • NDL 雑誌分類
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z53-G223
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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