Enzymatic Production of Highly Soluble Myricitrin Glycosides Using β-Galactosidase
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- SHIMIZU Ryosuke
- Fundamental Research Division, San-Ei Gen F.F.I. Corporation
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- SHIMABAYASHI Hiroshi
- Fundamental Research Division, San-Ei Gen F.F.I. Corporation
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- MORIWAKI Masamitsu
- Fundamental Research Division, San-Ei Gen F.F.I. Corporation
Bibliographic Information
- Other Title
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- Enzymatic Production of Highly Soluble Myricitrin Glycosides Using ベータ Galactosidase
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Abstract
Myricitrin, a botanical flavonol glycoside, could be a useful ingredient of functional foods, cosmetics, and medicines because of its high anti-oxidative activity. However, due to its insolubility in water, it has a limited range of use. To improve this solubility, we glycosylated myricitrin by an enzymatic transglycosylate reaction. Myricitrin was galactosylated by β-galactosidase from Bacillus circulans using lactose as a sugar donor. The reaction product was 480 times more soluble than myricitrin. Four myricitrin galactosides were isolated from the reaction products by column chromatography, and their molecular structures were identified by using ESI-MS, 1H-NMR, 13C-NMR, 1H–1H COSY, 1H-13C HMQC and 1H-13C HMBC analysis. The solubility of these four myricitrin galactosides was more than 3.9×103 fold that of myricitrin, and each had similar anti-oxidative activity to that of myricitrin.
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 70 (4), 940-948, 2006
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Details 詳細情報について
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- CRID
- 1390282681453281152
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- NII Article ID
- 10018532524
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- NII Book ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 7899900
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed