Improved Production of Enzymes, Which Are Expressed under the Pho Regulon Promoter, in the rmf Gene (encoding ribosome modulation factor) Disruptant of Escherichia coli
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Using a DNA macroarray, we investigated the effects of <I>rmf</I> gene (encoding ribosome modulation factor) disruption on gene expression profiles in <I>Escherichia coli</I>. This strain showed a phosphate-starvation-like response in gene expression even under phosphate sufficient conditions; significant upregulation of the Pho regulon genes was observed. Further, the production of alkaline phosphatase, a product of the Pho regulon gene, <I>phoA</I>, increased in the <I>rmf</I> disruptant under a Pi sufficient condition. Furthermore, production of PhoC acid phosphatase/nucleoside pyrophosphate phosphotransferase derived from <I>Morganella morganii</I> also increased significantly in the <I>rmf</I> disruptant. We concluded that host modification by the <I>rmf</I> gene disruption has potential benefit in industrial enzyme production using <I>Escherichia coli</I>.
- Agricultural and Biological Chemistry
Agricultural and Biological Chemistry 70(4), 949-957, 2006-04-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry