Improved Production of Enzymes, Which Are Expressed under the Pho Regulon Promoter, in the rmf Gene (encoding ribosome modulation factor) Disruptant of Escherichia coli

この論文にアクセスする

この論文をさがす

著者

抄録

Using a DNA macroarray, we investigated the effects of <I>rmf</I> gene (encoding ribosome modulation factor) disruption on gene expression profiles in <I>Escherichia coli</I>. This strain showed a phosphate-starvation-like response in gene expression even under phosphate sufficient conditions; significant upregulation of the Pho regulon genes was observed. Further, the production of alkaline phosphatase, a product of the Pho regulon gene, <I>phoA</I>, increased in the <I>rmf</I> disruptant under a Pi sufficient condition. Furthermore, production of PhoC acid phosphatase/nucleoside pyrophosphate phosphotransferase derived from <I>Morganella morganii</I> also increased significantly in the <I>rmf</I> disruptant. We concluded that host modification by the <I>rmf</I> gene disruption has potential benefit in industrial enzyme production using <I>Escherichia coli</I>.

収録刊行物

  • Bioscience, biotechnology, and biochemistry  

    Bioscience, biotechnology, and biochemistry 70(4), 949-957, 2006-04-23 

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

参考文献:  33件

参考文献を見るにはログインが必要です。ユーザIDをお持ちでない方は新規登録してください。

各種コード

  • NII論文ID(NAID)
    10018532563
  • NII書誌ID(NCID)
    AA10824164
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09168451
  • NDL 記事登録ID
    7900264
  • NDL 雑誌分類
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z53-G223
  • データ提供元
    CJP書誌  NDL  J-STAGE 
ページトップへ