Organ-Specific Transcription of Putative Flavonol Synthase Genes of Grapevine and Effects of Plant Hormones and Shading on Flavonol Biosynthesis in Grape Berry Skins
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In order to investigate the control mechanism of flavonol biosynthesis of grapevine, we obtained five genomic sequences (<I>FLS1</I> to <I>FLS5</I>) of putative flavonol synthase genes from <I>Vitis vinifera</I> cv. Cabernet Sauvignon. The mRNA of five <I>FLS</I>s accumulated in flower buds and flowers, while the mRNA of <I>FLS2</I>, <I>FLS4</I>, and <I>FLS5</I> accumulated in small berry skins and then decreased toward veraison. At the ripening stage, the mRNA of only <I>FLS4</I> and <I>FLS5</I> accumulated again. This change in mRNA accumulation did not contradict the flavonol accumulation in the berry skins. Shading of the berries completely inhibited the increase in flavonol content and mRNA accumulation of <I>FLS4</I>, but did not affect the mRNA accumulation of <I>FLS5</I>. The effects of light and plant hormones on flavonol accumulation were different from those on anthocyanin accumulation. Thus flavonol biosynthesis appears to be under a different control system from that of anthocyanin biosynthesis.
- Agricultural and Biological Chemistry
Agricultural and Biological Chemistry 70(3), 632-638, 2006-03-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry