Identification of Genes Affecting Lipid Content Using Transposon Mutagenesis in Saccharomyces cerevisiae

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著者

    • Noda Naomi NODA Naomi
    • Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST)
    • Tomita Nao [他] TOMITA Nao
    • Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST)
    • KIMURA Kazuyoshi
    • Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST)
    • HOSAKA Kohei
    • Department of Basic Sciences for Medicine, Gunma University School of Health Sciences

抄録

Genes involved in lipid accumulation were identified in <I>Saccharomyces cerevisiae</I> using transposon insertion mutagenesis. Five ORFs, such as <I>SNF2</I>, <I>IRA2</I>, <I>PRE9</I>, <I>PHO90</I>, and <I>SPT21</I> were found from the analysis of the insertion sites in transposon insertion mutants with higher lipid content. Since these ORFs are not directly involved in storage lipid biosynthesis, we speculate that they are involved in carbon fluxes into storage lipids in response to nutrient conditions. Lipid analysis of disruptants of these ORFs indicated that the Δ<I>snf2</I>, and Δ<I>ira2</I> disruptants had significantly higher lipid content. Cultivation in a nitrogen-limited medium increased the lipid content in all disruptants, among which the Δ<I>pre9</I> disruptant was the most sensitive to nitrogen limitation. We then focused on the Δ<I>snf2</I> disruptant due to its higher lipid content and its function as a regulator of phospholipid synthesis. Lipid class analysis indicated that triacylglycerol and free fatty acids contributed to the increase in total lipids of the Δ<I>snf2</I> disruptant. The addition of exogenous fatty acids was not so effective at increasing the lipid content in the Δ<I>snf2</I> disruptant as it was in the wild type. It should be noticed that exogenous free linoleic acid was much higher in the Δ<I>snf2</I> disruptant than in the wild type, as in the case of endogenous free fatty acids. In addition, the incorporation of exogenous fatty acids into cells increased in the disruptant, suggesting that fatty acid transporters were regulated by <I>SNF2</I>. The results suggest that metabolic fluxes into storage lipids, which are activated in the Δ<I>snf2</I> disruptant, is repressed by the incorporation of exogenous fatty acids. They provide new insight into the biosynthesis of storage lipids in yeast.

収録刊行物

  • Bioscience, biotechnology, and biochemistry  

    Bioscience, biotechnology, and biochemistry 70(3), 646-653, 2006-03-23 

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

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各種コード

  • NII論文ID(NAID)
    10018533386
  • NII書誌ID(NCID)
    AA10824164
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09168451
  • NDL 記事登録ID
    7871867
  • NDL 雑誌分類
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z53-G223
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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