Novel FAD-dependent glucose dehydrogenase to construct dioxygen-insensitive glucose biosensor

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  • TSUJIMURA Seiya
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
  • KOJIMA Shinki
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
  • KANO Kenji
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
  • IKEDA Tokuji
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
  • SATO Mika
    Ikeda Food Research Co., Ltd.
  • SANADA Hirokazu
    Ikeda Food Research Co., Ltd.
  • OMURA Hironori
    Ikeda Food Research Co., Ltd.

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タイトル別名
  • Novel FAD-Dependent Glucose Dehydrogenase for a Dioxygen-Insensitive Glucose Biosensor

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A novel FAD-dependent glucose dehydrogenase (FAD-GDH) was found and its enzymatic property for glucose sensing was characterized. FAD-GDH oxidized glucose in the presence of some artificial electron acceptors, except for O2, and exhibited thermostability, high substrate specificity and a large Michaelis constant for glucose. FAD-GDH was applied to an amperometric glucose sensor with Fe(CN)63− as a soluble mediator. The use of a relatively high concentration of Fe(CN)63− resulted in a good linearity between the current response and the glucose concentration, taking into account a large Michaelis constant for Fe(CN)63−. The glucose sensor was completely insensitive to O2 and responded linearly to glucose up to 30 mM. Compared to glucose, the response to other saccharides was negligible. The sensor can be stored at room temperature in a desiccator for at least one month without any change in the response or activity.

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