Biosynthesis of 2'-O-Methylmyxalamide D in the Myxobacterium Cystobacter fuscus : a Polyketide Synthase-Nonribosomal Peptide Synthetase System for the Myxalamide D Skeleton and a Methyltransferase for the Final O-Methylation
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The biosynthetic gene cluster for the polyene antifungal antibiotic, 2′-<I>O</I>-methylmyxalamide D, was cloned from myxobacterium <I>Cystobacter fuscus</I> AJ-13278. A sequence analysis of the 12.8-kb region in the gene cluster revealed the presence of two type I polyketide synthase genes, <I>mmxB</I> and <I>mmxC</I>. The involvement of these two genes in the biosynthesis of 2′-<I>O</I>-methylmyxalamide D was confirmed by a gene disruption experiments. In addition, an <I>S</I>-adenosylmethionine-dependent methyltransferase gene (<I>mmxM</I>) was found downstream of the gene cluster and demonstrated, by a gene disruption analysis, to be responsible for converting the known unmethylated precursor, myxalamide D, into 2′-<I>O</I>-methylmyxalamide D.
- Agricultural and Biological Chemistry
Agricultural and Biological Chemistry 70(3), 699-705, 2006-03-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry