Conjugational Transfer Kinetics of pLS20 between Bacillus subtilis in Liquid Medium
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pLS20-mediated conjugational transfer between <I>Bacillus subtilis</I> was investigated not on conventional solid media but in liquid culture. Detailed conjugational kinetics revealed that pLS20 transmission occurred at a limited cellular growth stage of both donor and recipient. Mutation of the recipient <I>recA</I> did not significantly interfere with the conjugational transfer process.
- Agricultural and Biological Chemistry
Agricultural and Biological Chemistry 70(3), 740-742, 2006-03-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry