Enzymatic Hydrolysis of Green Tea Seed Extract and Its Activity on 5α-Reductase Inhibition
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Two kaempferol glycosides were isolated from green tea seed extract (GTSE). After conducting a structure analysis, these two compounds were identified as kaempferol-3-<I>O</I>-[2-<I>O</I>-β-<small>D</small>-galactopyranosyl-6-<I>O</I>-α-<small>L</small>-rhamnopyranosyl]-β-<small>D</small>-glucopyranoside (compound 1) and kaempferol-3-<I>O</I>-[2-<I>O</I>-β-<small>D</small>-xylopyranosyl-6-<I>O</I>-α-<small>L</small>-rhanmopyranosyl]-β-<small>D</small>-glucopyranoside (compound 2). These two compounds were hydrolysed by <I>o</I>-glycolytic enzymes for the production of kaempferol. After performing several reactions, we found the optimum enzyme combination, a reaction with β-galactosidase and hesperidinase. Finally, we produced kaempferol of above 95% purity. The 5α-reductase inhibition activities of GTSE hydrolysate (GTSE-H) containing kaempferol were evaluated by the contact cell-based metabolic method using a stable HEK 293 cell line. GTSE-H showed a good inhibition effect on HEK 293 cell lines both type 1 and type 2 on 5α-reductase. Especially, GTSE-H inhibited type 2 with kaempferol content dependency. The results indicate that the inhibition activity of hydrolysate on 5α-reductase type 2 increases in accordance with kaempferol content.
- Agricultural and Biological Chemistry
Agricultural and Biological Chemistry 70(2), 387-394, 2006-02-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry