Enzymatic Hydrolysis of Green Tea Seed Extract and Its Activity on 5α-Reductase Inhibition

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Two kaempferol glycosides were isolated from green tea seed extract (GTSE). After conducting a structure analysis, these two compounds were identified as kaempferol-3-<I>O</I>-[2-<I>O</I>-β-<small>D</small>-galactopyranosyl-6-<I>O</I>-α-<small>L</small>-rhamnopyranosyl]-β-<small>D</small>-glucopyranoside (compound 1) and kaempferol-3-<I>O</I>-[2-<I>O</I>-β-<small>D</small>-xylopyranosyl-6-<I>O</I>-α-<small>L</small>-rhanmopyranosyl]-β-<small>D</small>-glucopyranoside (compound 2). These two compounds were hydrolysed by <I>o</I>-glycolytic enzymes for the production of kaempferol. After performing several reactions, we found the optimum enzyme combination, a reaction with β-galactosidase and hesperidinase. Finally, we produced kaempferol of above 95% purity. The 5α-reductase inhibition activities of GTSE hydrolysate (GTSE-H) containing kaempferol were evaluated by the contact cell-based metabolic method using a stable HEK 293 cell line. GTSE-H showed a good inhibition effect on HEK 293 cell lines both type 1 and type 2 on 5α-reductase. Especially, GTSE-H inhibited type 2 with kaempferol content dependency. The results indicate that the inhibition activity of hydrolysate on 5α-reductase type 2 increases in accordance with kaempferol content.

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  • Bioscience, biotechnology, and biochemistry  

    Bioscience, biotechnology, and biochemistry 70(2), 387-394, 2006-02-23 

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

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各種コード

  • NII論文ID(NAID)
    10018534138
  • NII書誌ID(NCID)
    AA10824164
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09168451
  • NDL 記事登録ID
    7833443
  • NDL 雑誌分類
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z53-G223
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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