Enzymatic Hydrolysis of Green Tea Seed Extract and Its Activity on 5α-Reductase Inhibition
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- PARK Jun-Seong
- Skin Research Institute, AMOREPACIFIC R&D Center
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- YEOM Myeong-Hoon
- Skin Research Institute, AMOREPACIFIC R&D Center
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- PARK Won-Seok
- Skin Research Institute, AMOREPACIFIC R&D Center
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- JOO Kyung-Mi
- Skin Research Institute, AMOREPACIFIC R&D Center
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- RHO Ho-Sik
- Skin Research Institute, AMOREPACIFIC R&D Center
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- KIM Duck Hee
- Skin Research Institute, AMOREPACIFIC R&D Center
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- CHANG Ih Seop
- Skin Research Institute, AMOREPACIFIC R&D Center
書誌事項
- タイトル別名
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- Enzymatic Hydrolysis of Green Tea Seed Extract and Its Activity on 5.ALPHA.-Reductase Inhibition
- Enzymatic Hydrolysis of Green Tea Seed Extract and Its Activity on 5 アルファ Reductase Inhibition
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抄録
Two kaempferol glycosides were isolated from green tea seed extract (GTSE). After conducting a structure analysis, these two compounds were identified as kaempferol-3-O-[2-O-β-D-galactopyranosyl-6-O-α-L-rhamnopyranosyl]-β-D-glucopyranoside (compound 1) and kaempferol-3-O-[2-O-β-D-xylopyranosyl-6-O-α-L-rhanmopyranosyl]-β-D-glucopyranoside (compound 2). These two compounds were hydrolysed by o-glycolytic enzymes for the production of kaempferol. After performing several reactions, we found the optimum enzyme combination, a reaction with β-galactosidase and hesperidinase. Finally, we produced kaempferol of above 95% purity. The 5α-reductase inhibition activities of GTSE hydrolysate (GTSE-H) containing kaempferol were evaluated by the contact cell-based metabolic method using a stable HEK 293 cell line. GTSE-H showed a good inhibition effect on HEK 293 cell lines both type 1 and type 2 on 5α-reductase. Especially, GTSE-H inhibited type 2 with kaempferol content dependency. The results indicate that the inhibition activity of hydrolysate on 5α-reductase type 2 increases in accordance with kaempferol content.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 70 (2), 387-394, 2006
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206475752192
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- NII論文ID
- 10018534138
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 7833443
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- 抄録ライセンスフラグ
- 使用不可