Stimulatory Effect of Lactic Acid Bacteria from Commercially Available Nozawana-zuke Pickle on Cytokine Expression by Mouse Spleen Cells
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We investigated the effect of lactic acid bacteria (LAB) isolated from eight samples of commercially available Nozawana-zuke, a traditional Japanese pickle, on cytokine expression by mouse spleen cell cultures. The 12 isolated strains of LAB (Nz1–Nz12), which were identified as genus <I>Lactobacillus</I> or <I>Leuconostoc</I> by the API50CHL test, enhanced the expression of interferon (IFN)-γ with 6 h of culture. Ten of these 12 LAB, particularly Nz8, enhanced interleukin (IL)-12 p40 expression. The actinase E- and Benzonase-treated or untreated cell wall fraction of Nz8 enhanced both IFN-γ and IL-12 p40 expression, while the cell plasma fraction had little effect. In the presence of anti-toll like receptor 4 antibody, the effect of the cell wall fraction of Nz8 was significantly abrogated. These results suggest that some LAB from Nozawana-zuke have a T helper 1-type immunoenhancing effect.
- Agricultural and Biological Chemistry
Agricultural and Biological Chemistry 70(2), 411-417, 2006-02-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry