Blood Pressure-Depressing Activity of a Peptide Derived from Silkworm Fibroin in Spontaneously Hypertensive Rats

• IGARASHI Kiharu

  Department of Bioresource Engineering, Faculty of Agriculture, Yamagata University

• YOSHIOKA Kosuke

  Department of Bioresource Engineering, Faculty of Agriculture, Yamagata University

• MIZUTANI Kenji

  Research Center, Maruzen Pharmaceuticals Co., Ltd.

• MIYAKOSHI Masazumi

  Research Center, Maruzen Pharmaceuticals Co., Ltd.

• MURAKAMI Toshiyuki

  Research Center, Maruzen Pharmaceuticals Co., Ltd.

• AKIZAWA Toshifumi

  Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Setsunan University

Abstract

Peptides showing inhibitory activity against the angiotensin I-converting enzyme (ACE) were investigated from the fibroin fraction of discarded silk fabric. Fibroin, which was hydrolyzed with alcalase after partial hydrolysis with hot aqueous 40% CaCl₂, released two major active peptides showing ACE-inhibitory activity. The two peptides were identified as glycyl-valyl-glycyl-tyrosine (GVGY) and glycyl-valyl-glycyl-alanyl-glycyl-tyrosine (GVGAGY) by analyses with a protein sequencer and LC/MS/MS. GVGY, whose ACE-inhibitory activity has not previously been reported, showed a blood pressure-depressing effect on spontaneously hypertensive rat (SHR).

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 70 (2), 517-520, 2006

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• fibroin
• peptide
• blood pressure
• spontaneously hypertensive rat (SHR)
• angiotensin I-converting enzyme

Details

• CRID: 1390282681452502016
• NII Article ID: 10018534632
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.70.517
• COI: 1:CAS:528:DC%2BD28XisVSqsL0%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL BIB ID: 7833629
• PubMed: 16495671
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7833629
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.70.517
• Web Site: https://search.jamas.or.jp/link/ui/2007048543
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed