Yeast Two-Hybrid Detection Systems That Are Highly Sensitive to a Certain Kind of Endocrine Disruptors
We tested the effects of several combinations of bait and fish components of the yeast two-hybrid detection system for estrogenic activity. A combination of the full-length human estrogen receptor α with the nuclear receptor-binding domain of co-activator steroid receptor co-activator-1 (SRC-1) or transcriptional intermediate factor-2 (TIF-2) was most effective for estrogen-dependent induction of the chromosome-integrated UAS<SUB>GAL</SUB>-<I>CYC1</I><SUP>p</SUP>-<I>lacZ</I> reporter construct among the two-hybrid systems so far tested.
- Bioscience, biotechnology, and biochemistry
Bioscience, biotechnology, and biochemistry 70(2), 521-524, 2006-02-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry