Transcriptome Analysis Reveals Global Expression Changes in an Industrial L-Lysine Producer of Corynebacterium glutamicum
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Toward the elucidation of advanced mechanisms of <small>L</small>-lysine production by <I>Corynebacterium glutamicum</I>, a highly developed industrial strain B-6 was analyzed from the viewpoint of gene expression. Northern blot analysis showed that the <I>lysC</I> gene encoding aspartokinase, the key enzyme of <small>L</small>-lysine biosynthesis, was up-regulated by several folds in strain B-6, while no repression mechanism exists in <small>L</small>-lysine biosynthesis of this bacterium. To analyze the underlying mechanisms of the up-regulation, we compared the transcriptome between strain B-6 and its parental wild-type, finding that not only <I>lysC</I> but also many other amino acid-biosynthetic genes were up-regulated in the producer. These results suggest that a certain global regulatory mechanism is involved in the industrial levels of <small>L</small>-lysine production.
- Agricultural and Biological Chemistry
Agricultural and Biological Chemistry 70(2), 546-550, 2006-02-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry