Simultaneous Determination of Residual Veterinary Drugs in Bovine, Porcine, and Chicken Muscle Using Liquid Chromatography Coupled with Electrospray Ionization Tandem Mass Spectrometry
A simple and rapid method using liquid chromatography coupled with electrospray ionization tandem mass spectrometry (LC/MS/MS) for the simultaneous determination of 130 veterinary drugs and their metabolites in bovine, porcine, and chicken muscle was developed. The drugs (1 to 10 ng/g, in muscle) were extracted from bovine, porcine, or chicken muscles with acetonitrile-methanol (95:5, v/v), and the extracts were delipidated with <I>n</I>-hexane saturated with acetonitrile. The extracts were evaporated, dissolved with methanol, analyzed by liquid chromatography with gradient elution on a C<SUB>18</SUB> column, and determined by electrospray ionization tandem mass spectrometry. The detection limits ranged from 0.03 to 3 ng/g. The quantitation limits ranged from 0.1 to 10 ng/g. One hundred eleven, 122, and 123 drugs from bovine, porcine, and chicken muscle respectively showed recoveries between 70 and 110%.
- Bioscience, biotechnology, and biochemistry
Bioscience, biotechnology, and biochemistry 70(1), 54-65, 2006-01-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry