Analysis of Tissue-Specific Expression of Human Type 2 Collagen cDNA Driven by Different Sizes of the Upstream Region of the β-Casein Promoter
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- NARUSE Kenji
- Research Center for Transgenic and Cloned Pigs, Chungnam National University
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- YOO Seung Kwon
- Division of Biotechnology and Genetic Engineering, Korea University
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- KIM Sun Myoung
- Department of Animal Science and Technology, Seoul National University
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- CHOI Yun Jaie
- Department of Animal Science and Technology, Seoul National University
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- LEE Hong Mie
- Department of Food Science and Nutrition, Daejin University
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- JIN Dong Il
- Research Center for Transgenic and Cloned Pigs, Chungnam National University
書誌事項
- タイトル別名
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- Analysis of Tissue-Specific Expression of Human Type II Collagen cDNA Driven by Different Sizes of the Upstream Region of the .BETA.-Casein Promoter
- Analysis of Tissue Specific Expression of Human Type 2 Collagen cDNA Driven by Different Sizes of the Upstream Region of the ベータ Casein Promoter
- Analysis of Tissue-Specific Expression of Human Type II Collagen cDNA Driven by Different Sizes of the Upstream Region of the β-Casein Promoter
この論文をさがす
抄録
To investigate the ability of 1.8 kb or 3.1 kb bovine β-casein promoter sequences for the expression regulation of transgene in vivo, transgenic mice were produced with human type II collagen gene fused to 1.8 kb and 3.1 kb of bovine β-casein promoter by DNA microinjection. Five and three transgenic founder mice were produced using transgene constructs with 1.8 kb and 3.1 kb of bovine β-casein promoters respectively. Founder mice were outbred with the wild type to produce F1 and F2 progenies. Total RNAs were extracted from four tissues (mammary gland, liver, kidney, and muscle) of female F1 transgenic mice of each transgenic line following parturition. RT-PCR and Northern blot analysis revealed that the expression level of transgene was variable among the transgenic lines, but transgenic mice containing 1.8 kb of promoter sequences exhibited more leaky expression of transgene in other tissues compared to those with 3.1 kb promoter. Moreover, Western blot analysis of transgenic mouse milk showed that human type II collagen proteins secreted into the milk of lactating transgenic mice contained 1.8 kb and 3.1 kb of bovine β-casein promoter. These results suggest that promoter sequences of 3.1 kb bovine β-casein gene can be used for induction of mammary gland-specific expression of transgenes in transgenic animals.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 70 (1), 93-98, 2006
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206475426944
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- NII論文ID
- 10018535128
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 7791290
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
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- 抄録ライセンスフラグ
- 使用不可